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Journal / Neuroscience / The Culture of Primary Motor and Sensory Neurons…
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Neuroscience

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JoVE Journal Neuroscience
The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds
 

The Culture of Primary Motor and Sensory Neurons in Defined Media on Electrospun Poly-L-lactide Nanofiber Scaffolds

Article DOI: 10.3791/2389-v • 16:03 min • February 15th, 2011
February 15th, 2011 •
1, 2, 3, 3, 3, 3, 3, 1,3,4
1Department of Biomedical Engineering, University of Michigan, 2State Key Laboratory of Bioelectronics, Southeast University, 3Department of Neurology, University of Michigan, 4Geriatric Research, Education and Clinical Center, Veterans Affairs Ann Arbor Health System

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Aligned electrospun fibers direct the growth of neurons in vitro and are a potential component of nerve regeneration scaffolds. We describe a procedure for preparing electrospun fiber substrates and the serum-free culture of primary rat E15 sensory (DRG) and motor neurons. Visualization of neurons by immunocytochemistry is also included.

Tags

Culture Primary Motor Neurons Sensory Neurons Defined Media Electrospun Nanofiber Scaffolds Electrospinning Technique Alignment Random Fibers Biodegradable Polymers Poly-L-lactic Acid (PLLA) Tissue Engineering Nerve Regeneration Primary Cell Culture System Biomaterials Dorsal Root Ganglia Explants Dissociated Neurons
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