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Criopreservação de tecido cortical Blocos de Geração de culturas altamente enriquecido Neuronal
Chapters
- 00:00Title
- 01:15Material Preparation
- 02:41Tissue Preparation
- 04:20Cryopreservation
- 06:05Thaw and Culture
- 08:42Conclusion
Aqui, nós descrevemos um método eficiente para criopreservação e descongelamento de blocos de tecido cortical do cérebro para gerar altamente enriquecido culturas neuronal. Este protocolo simples, fornece flexibilidade para a geração posterior de culturas de células neuronais, astrócitos, e neuronal precursor.
Tags
CryopreservationNeuronal CulturesFreezing MediumDimethyl Sulfoxide (DMSO)Hank’s Buffered Salt Solution (HBSS)CryovialsRate-controlled Freezing ContainerPost-thaw ProcessingDissociationNeuronal-enriched CulturesNeuritic GrowthImmunocytochemical StainingAstrocytic MarkerGlial Fibrillary Acidic Protein (GFAP)Neuronal MarkerBeta-tubulin Class IIINeural Precursor Cell CulturesNeurospheresDifferentiating Conditions
