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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
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Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures
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November 11, 2010
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Here, we describe a method for efficient cryopreservation and thawing of cortical brain tissue blocks to generate highly enriched neuronal cultures. This simple protocol provides flexibility for later generation of neuronal, astrocyte, and neuronal precursor cell cultures.
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Rahman, A. S., Parvinjah, S., Hanna, M. A., Helguera, P. R., Busciglio, J. Cryopreservation of Cortical Tissue Blocks for the Generation of Highly Enriched Neuronal Cultures. J. Vis. Exp. (45), e2384, doi:10.3791/2384 (2010).
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