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重组逆转录病毒的生产和B细胞的感染
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JoVE 신문 면역학 및 감염병학
Recombinant Retroviral Production and Infection of B Cells
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重组逆转录病毒的生产和B细胞的感染

DOI:
10.3791/2371-v

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09:19 min

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February 18, 2011

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, ,
1Department of Microbiology and Immunology,Columbia University College of Physicians and Surgeons, 2Herbert Irving Comprehensive Cancer Center,Columbia University

Chapters

  • 00:05Title
  • 01:24Splenic B-Lymphocyte Isolation and Stimulation
  • 05:42Transfection of Producer Cells
  • 07:26Infection of Stimulated Splenic B cells
  • 08:12Flow Cytometry Analysis
  • 08:40Rescue of AID-Deficiency by Transduction of AID-Deficient B Cells with a Recombinant Retrovirus
  • 08:57Conclusion

Summary

자동 번역

자동 번역

一个有效率的制度中的一个基因的结构和功能分析<em>体外</em>脾B淋巴细胞的文化描述。这种方法需要利用一个辅助的自由，ecotrophic包装细胞系的重组逆转录病毒的生产。稳定，遗传兴趣在初级淋巴细胞基因的表达，是实现领导在接受类开关重组的B细胞表面抗体的生成。

Tags

Recombinant Retroviral ProductionB CellsTransgenic ExpressionReverse Genetic ApproachHeterologous Expression SystemPhenotype AnalysisGene Over-expressionHematopoetic SystemMouse GeneticsHuman Genes And DiseasesSynteny ConservationGene DisruptionsAllelic ReplacementTransgenic Mouse CreationCell Culture Model

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