Journal
/
면역학 및 감염병학
/
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors
JoVE 신문
면역학 및 감염병학
This content is Free Access.
JoVE 신문 면역학 및 감염병학
Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors

DOI:
10.3791/2363-v

•

07:12 min

•

November 24, 2010

•

, , , ,
1Microbiology, Immunology, and Pathology,Colorado State University

Chapters

  • 00:05Title
  • 01:38per os Infection of Mosquitoes
  • 03:28Monitoring Infection of Mosquitoes
  • 04:18Transmission Assay (Forced salivation to demonstrate virus transmission)
  • 05:30Alphavirus Infection of Mosquito Cell Culture and the Adult Female Mosquito
  • 06:48Conclusion

Summary

자동 번역

자동 번역

Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.

Tags

Alphavirus Transducing SystemVisualizing InfectionMosquito VectorsGene ExpressionMosquito-borne RNA VirusesAlphavirus GenusCDNA ClonesSingle-stranded RNA GenomesStructural ProteinsGene InsertionViral Subgenomic RNA Initiation SitePromoterStable Protein ExpressionRNAiGene Silencing

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Visualizing Dengue Virus through Alexa Fluor Labeling

piggyBac Transposon System Modification of Primary Human T Cells

Culturing Microglia from the Neonatal and Adult Central Nervous System

Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species

A Simple and Efficient Approach to Construct Mutant Vaccinia Virus Vectors

Visualizing the Effects of Sputum on Biofilm Development Using a Chambered Coverglass Model

Visualizing Leukocyte Rolling and Adhesion in Angiotensin II-Infused Mice: Techniques and Pitfalls

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Isolating Central Nervous System Tissues and Associated Meninges for the Downstream Analysis of Immune cells

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice

Read Article