Methods for using alphavirus transducing systems to express fluorescent reporters in vitro and in adult mosquitoes are described. This technique may be adapted to express any protein of interest in lieu of or in addition to a reporter.
Phillips, A., Mossel, E., Sanchez-Vargas, I., Foy, B., Olson, K. Alphavirus Transducing System: Tools for Visualizing Infection in Mosquito Vectors. J. Vis. Exp. (45), e2363, doi:10.3791/2363 (2010).