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Выделение головного и спинного мозга одноядерных клеток Использование Перколла Градиенты
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:27Preparation of Reagents
- 02:09Tissue Collection and Homogenization
- 02:56Gradient Set Up
- 05:56Antibody Staining
- 08:08Representative Analysis by Flow Cytometry
- 09:29Conclusion
Tags
IsolationBrainSpinal CordMononuclear CellsPercoll GradientsImmune CellsCentral Nervous SystemInfectionTraumaAutoimmunityNeurodegenerationPhenotypeEffector FunctionsHistochemical ApproachesInfiltrating CellsCNS PathologyIn-situ HistochemistryImmunofluorescent Staining TechniquesAntibodiesImmune Cell SubtypesHistological ApproachesFlow CytometryCompositionCellular Suspensions
