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Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients
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February 02, 2011
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The current article describes a rapid protocol to efficiently isolate mononuclear cells from brain and spinal cord tissues that can be effectively utilized for flow cytometric analyses.
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IsolationBrainSpinal CordMononuclear CellsPercoll GradientsImmune CellsCentral Nervous SystemInfectionTraumaAutoimmunityNeurodegenerationPhenotypeEffector FunctionsHistochemical ApproachesInfiltrating CellsCNS PathologyIn-situ HistochemistryImmunofluorescent Staining TechniquesAntibodiesImmune Cell SubtypesHistological ApproachesFlow CytometryCompositionCellular Suspensions
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Cite this Article
Pino, P. A., Cardona, A. E. Isolation of Brain and Spinal Cord Mononuclear Cells Using Percoll Gradients. J. Vis. Exp. (48), e2348, doi:10.3791/2348 (2011).
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