JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Establishing a Co-Culture of Interneurons with Periventricular Endothelial Cells
내레이션 대본
To perform the co-culture assay, suspend 30,000 GABAergic interneurons and 30,000 human periventricular endothelial cells in 70 microliters of co-culture medium. Then, seed this cell solution inside a one-well insert compartment. After 48 hours, remove the insert. Incubate the co-culture at 37 degrees Celsius and 5% carbon dioxide for five days.
