Establishing a Co-Culture of Interneurons with Periventricular Endothelial Cells

To perform the co-culture assay, suspend 30,000 GABAergic interneurons and 30,000 human periventricular endothelial cells in 70 microliters of co-culture medium. Then, seed this cell solution inside a one-well insert compartment. After 48 hours, remove the insert. Incubate the co-culture at 37 degrees Celsius and 5% carbon dioxide for five days.