JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:03Thawing, Seeding, and Expanding Arctic Ground Squirrel Neural Stem Cells (agsNSC)
- 01:05Preparing poly-L-ornithine-coated Flasks
- 01:56Thawing and Seeding agsNSC
- 03:39Expanding agsNSC
- 04:11Trypsinization of Cells
- 05:27Inoculating Well Plates for Differentiation
- 07:25Maintenance of Neurons
- 08:09Results of Growth and Differentiation of agsNSC
- 09:29Conclusion
Tags
GrowthDifferentiationAdult HippocampalArctic Ground SquirrelNeural Stem CellsHibernationIschemic InjuryBrainOxygen-glucose DeprivationNeuronsProteinsGene TargetsTherapiesIschemic IndicationsCold ToleranceProliferationCultureTUJ1-positive NeuronsToxic ChemicalsNeurogenesisMEK/ERK Signaling PathwayPI3K/Akt Signaling Pathway
