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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
Chapters
- 00:00Title
- 00:54Introduction
- 01:18Static Co-culture Biofilm Model
- 03:41Flow Cell Co-culture Biofilm Model
- 08:36Representative Results
- 10:52Conclusion
This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.
Tags
Co-culture ModelsPseudomonas Aeruginosa BiofilmsHuman Airway CellsCFBE CellsStatic Co-culture Biofilm ModelFlow Cell Co-culture Biofilm ModelArginineBacterial BiofilmsEpithelial CellsBiofilm DevelopmentGlass CoverslipPeristaltic PumpFresh MediumVisualizationGreen Fluorescent Protein (GFP)Cystic Fibrosis (CF) Lung
