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Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

Co-culture Models of Pseudomonas aeruginosa Biofilms Grown on Live Human Airway Cells

21,892 Views

11:21 min

October 06, 2010

DOI:

11:21 min
October 06, 2010

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概要

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This paper describes different methods of growing Pseudomonas aeruginosa biofilms on cultured human airway epithelial cells. These protocols can be adapted to study different aspects of biofilm formation, including visualization of the biofilm, staining of the biofilm, measuring the colony forming units (CFU) of the biofilm, and studying biofilm cytotoxicity.

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