Encyclopedia of Experiments
Biological Techniques
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Encyclopedia of Experiments Biological Techniques
Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

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Before staining the cells, load the sample vial into an automated processor for slide preparation. When the slides are ready, dip the samples into 95% ethanol, 10 times, for 1 second per dip, followed by 10 dips in water, for 1 second per dip.

Next, submerge the slides in hematoxylin for 10 to 60 seconds, followed by a second water-ethanol wash. After the last ethanol immersion, submerge the slides in Eosin Azure for 1 to 3 minutes, followed by another water ethanol rinse. Then, dip the slides in xylene; until the samples become clear.

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