Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

Before staining the cells, load the sample vial into an automated processor for slide preparation. When the slides are ready, dip the samples into 95% ethanol, 10 times, for 1 second per dip, followed by 10 dips in water, for 1 second per dip.

Next, submerge the slides in hematoxylin for 10 to 60 seconds, followed by a second water-ethanol wash. After the last ethanol immersion, submerge the slides in Eosin Azure for 1 to 3 minutes, followed by another water ethanol rinse. Then, dip the slides in xylene; until the samples become clear.