Journal
/
면역학 및 감염병학
/
弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Toxoplasma gondii Cyst Wall Formation in Activated Bone Marrow-derived Macrophages and Bradyzoite Conditions
Please note that all translations are automatically generated. Click here for the English version.

弓形虫囊肿壁形成激活骨髓衍生的巨噬细胞和Bradyzoite条件

DOI:
10.3791/2091-v

•

09:56 min

•

August 12, 2010

•

, ,
1Department of Medical Microbiology and Immunology,University of Wisconsin

Chapters

  • 00:04Title
  • 00:44Introduction
  • 01:10Preparation of HFF-coated Coverslips
  • 02:37Infecting Cells
  • 03:57Detection of Bradyzoites
  • 05:48Inducing Cyst Wall Formation
  • 09:08Representative Results
  • 09:30Conclusion

Summary

자동 번역

자동 번역

弓形虫囊肿的形式，在应对环境压力，可在组织培养模式模仿的转换。本视频演示了技术审查囊肿壁的形成，激活骨髓来源的巨噬细胞或改变成纤维细胞生长介质的pH值。

Tags

Toxoplasma GondiiCyst Wall FormationBone Marrow-derived MacrophagesBradyzoite ConditionsObligate Intracellular ParasiteTachyzoiteEncysted FormSignaling ProcessChronic Stage Of InfectionCO2 StarvationHigh PH MediumInterferon Gamma (IFNγ)Dolichos Biflorus Agglutinin (DBA)Fluorescently Labeled DBAHuman Foreskin Fibroblasts (HFFs)Murine Bone Marrow-derived Macrophages (BMMs)Lipopolysaccharide (LPS)High PH Growth Medium

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

取得高纯度弓形虫卵囊

与预先定义的T细胞受体特异性Transnuclear小鼠对弓形虫获得通过体细胞核移植

小鼠骨髓来源的树突状细胞与跟踪研究的Qdot纳米晶的生成及标签

遗传筛选隔离弓形虫宿主细胞的出口突变

使用骨髓源性巨噬细胞巨噬细胞极化的调查

遗传操纵<em>Δku80</em>株功能基因组分析<em>弓形虫</em

量<em在体外</em>激活的主人微血管内皮细胞在静态条件下测量的检测，以中性粒细胞粘附

骨髓衍生的巨噬细胞产

隔离和小鼠骨髓的静脉注射单核细胞衍生

正向遗传学屏风使用巨噬细胞识别<em>弓形虫</em>基因的重要的抗IFN-γ依赖性细胞免疫自主

Read Article