Journal
/
생물학
/
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

22,145 Views

•

15:55 min

•

August 04, 2010

DOI:

10.3791/2079-v

DOI:
10.3791/2079-v

•

15:55 min
August 04, 2010

•

4 Views
, , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Department of Medicine and Genetics,Harvard Medical School

Summary

Automatically generated

We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.

Explore More Videos

Intravenous MicroinjectionsZebrafish LarvaeAcute Kidney InjuryGentamicinNephrotoxicantBloodstreamFish Per HourExperimental StudiesAngiographyProtocolKidney FunctionFixationImmunohistochemistryKidney Tubule MarkerPlastic EmbeddingSectioning

Read Article

Cite this Article

Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., Hukriede, N. A. Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury. J. Vis. Exp. (42), e2079, doi:10.3791/2079 (2010).

Download .ris file

Copy

Share Video

.

Copy