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Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury
JoVE 杂志
生物学
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JoVE 杂志 生物学
Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

22,145 Views

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15:55 min

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August 04, 2010

DOI:

10.3791/2079-v

DOI:
10.3791/2079-v

•

15:55 min
August 04, 2010

•

4 Views
, , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Department of Medicine and Genetics,Harvard Medical School

Summary

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We describe a technique of microinjecting the aminoglycoside, gentamicin, into 2 days post-fetilization (dpf) zebrafish larvae to induce acute kidney injury (AKI). We also describe a method for whole mount immunohistochemistry, plastic embedding and sectioning of zebrafish larvae to visualize the AKI mediated damage.

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Intravenous MicroinjectionsZebrafish LarvaeAcute Kidney InjuryGentamicinNephrotoxicantBloodstreamFish Per HourExperimental StudiesAngiographyProtocolKidney FunctionFixationImmunohistochemistryKidney Tubule MarkerPlastic EmbeddingSectioning

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Cite this Article

Cianciolo Cosentino, C., Roman, B. L., Drummond, I. A., Hukriede, N. A. Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury. J. Vis. Exp. (42), e2079, doi:10.3791/2079 (2010).

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