This content is Free Access.
24,380 Views
•
11:14 min
•
July 26, 2010
DOI:
1Department of Pathology, Hershey Medical Center,Pennsylvania State University, 2Department of Chemistry,Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center,Pennsylvania State University, 4Materials Research Institute,Pennsylvania State University
Summary
Automatically generated
SELEX协议包括多轮选拔，其中每一个需要结合的配体，这反过来又需要固定的引物序列，侧翼的随机库地区的再生。这些固定的引物序列，可以干扰与选择过程（误报和底片）。在这里，我们目前无底漆的协议。
Explore More Videos
Primer-Free Aptamer SelectionRandom DNA LibraryAptamersOligonucleotidesDNARNAAffinitiesAntibodiesIn Vitro Selection ProcessSystematic Evolution Of Ligands By EXponential Enrichment (SELEX)TargetsSmall MoleculesProteinsMacromoleculesIn Vivo Diagnostic ApplicationsTherapeutic ApplicationsSpecificitySynthesizedProcessing ConditionsImmunogenicitySizeNucleotides (nt)Nucleic Acid LibrariesRandomized RegionsPrimer SitesSelection Process ComplicationsBioinformatics Approaches
Read Article
Cite this Article
Pan, W., Xin, P., Patrick, S., Dean, S., Keating, C., Clawson, G. Primer-Free Aptamer Selection Using A Random DNA Library. J. Vis. Exp. (41), e2039, doi:10.3791/2039 (2010).
Copy