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Primer-Free Aptamer Selection Using A Random DNA Library
 

Primer-Free Aptamer Selection Using A Random DNA Library

Article DOI: 10.3791/2039-v • 11:14 min • July 26th, 2010
July 26th, 2010 •
1, 1, 1, 2, 2, 3,4
1Department of Pathology, Hershey Medical Center, Pennsylvania State University, 2Department of Chemistry, Pennsylvania State University, 3Departments of Pathology, and Biochemistry and Molecular Biology, Hershey Medical Center, Pennsylvania State University, 4Materials Research Institute, Pennsylvania State University

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Summary
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SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.

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Primer-Free Aptamer Selection Random DNA Library Aptamers Oligonucleotides DNA RNA Affinities Antibodies In Vitro Selection Process Systematic Evolution Of Ligands By EXponential Enrichment (SELEX) Targets Small Molecules Proteins Macromolecules In Vivo Diagnostic Applications Therapeutic Applications Specificity Synthesized Processing Conditions Immunogenicity Size Tissues Penetration Nucleotides (nt) Nucleic Acid Libraries Randomized Regions Primer Sites Selection Process Complications Bioinformatics Approaches
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