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Primer-Free Aptamer Selection Using A Random DNA Library
章
- 00:04標題
- 00:12Introduction
- 01:39Preparation of PF Library-DNA Fragments
- 03:26Preparation of S100B-bound Beads
- 04:55Selection & Recovery of Aptamers
- 06:40TOPO Cloning and Sequencing
- 07:17Sandwich Binding Assays
- 09:11Representative Results
- 10:35Conclusion
SELEX protocols comprise multiple rounds of selection, each of which require regeneration of bound ligands, which in turn require fixed primer sequences flanking the random library regions. These fixed primer sequences can interfere with the selection process (false positives and negatives). Here we present a primer-free protocol.
タグ
Primer-Free Aptamer SelectionRandom DNA LibraryAptamersOligonucleotidesDNARNAAffinitiesAntibodiesIn Vitro Selection ProcessSystematic Evolution Of Ligands By EXponential Enrichment (SELEX)TargetsSmall MoleculesProteinsMacromoleculesIn Vivo Diagnostic ApplicationsTherapeutic ApplicationsSpecificitySynthesizedProcessing ConditionsImmunogenicitySizeNucleotides (nt)Nucleic Acid LibrariesRandomized RegionsPrimer SitesSelection Process ComplicationsBioinformatics Approaches
