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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Large Protein Complexes by Structural Mass Spectrometry

23,720 Views

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15:35 min

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June 19, 2010

DOI:

10.3791/1954-v

DOI:
10.3791/1954-v

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15:35 min
June 19, 2010

•

10 Views
, ,
1Department of Biological Chemistry,Weizmann Institute of Science

Summary

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Mass spectrometry has proven to be a valuable tool for analyzing large protein complexes. This method enables insights into the composition, stoichiometry and overall architecture of multi-subunit assemblies. Here, we describe, step-by-step, how to perform a structural mass spectrometry analysis, and characterize macromolecular structures.

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Cite this Article

Kirshenbaum, N., Michaelevski, I., Sharon, M. Analyzing Large Protein Complexes by Structural Mass Spectrometry. J. Vis. Exp. (40), e1954, doi:10.3791/1954 (2010).

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