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Analyzing Large Protein Complexes by Structural Mass Spectrometry
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生物学
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JoVE Journal 生物学
Analyzing Large Protein Complexes by Structural Mass Spectrometry

Analyzing Large Protein Complexes by Structural Mass Spectrometry

DOI:
10.3791/1954-v

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15:35 min

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June 19, 2010

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, ,
1Department of Biological Chemistry,Weizmann Institute of Science

章

  • 00:00標題
  • 00:46Introduction
  • 01:18Preparation of Gold-coated Capillaries for Nanoflow Electrospray Ionization
  • 04:12Calibrating the Mass Spectrometer for High Mass Measurements
  • 07:47MS Analysis of Intact Protein Complexes
  • 10:45Tandem Mass Spectrometry: Dissociating Protein Complexes
  • 13:08Representative Results of Large Protein Complexes Analysis by Mass Spectrometry
  • 15:09Conclusion

概要

自動翻訳

自動翻訳

Mass spectrometry has proven to be a valuable tool for analyzing large protein complexes. This method enables insights into the composition, stoichiometry and overall architecture of multi-subunit assemblies. Here, we describe, step-by-step, how to perform a structural mass spectrometry analysis, and characterize macromolecular structures.

タグ

Large Protein ComplexesStructural Mass SpectrometryBiological ProcessesProtein OrganizationMacromolecular ComplexesMass Spectrometry (MS)Protein CompositionSubunit StoichiometryStructural TopologyHigh SensitivityLow Sample RequirementReal-time AnalysisSeparate PopulationsMixture Analysis

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