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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

11,890 Views

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20:47 min

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August 13, 2009

DOI:

10.3791/1561-v

DOI:
10.3791/1561-v

•

20:47 min
August 13, 2009

•

7 Views
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1Department of Microbiology, Medical School,University of Minnesota, 2Department of Veterinary and Biomedical Sciences,University of Minnesota

Summary

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A technique combining in situ tetramer staining and in situ hybridization (ISTH) enables visualization, mapping and analysis of the spatial proximity of virus-specific CD8+ T cells to their virus-infected targets, and determination of the quantitative relationships between these immune effectors and targets to infection outcomes.

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Virus-specific CD8+ T CellsVirus-infected CellsVisualizeIn SituImmune Effector CellsImmune DefensesInfectionSpatial ProximityQuantitative RelationshipsClearanceChronic Persistent InfectionsIn Situ Tetramer StainingIn Situ HybridizationViral-RNA+ CellsBattle Between Immune Defenses And InfectionISTH (in Situ Tetramer Staining And In Situ Hybridization)Mapping Of LocationsE:T RatiosTiming And Magnitude Of The Immune Response

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Cite this Article

Li, Q., J. Skinner, P., Duan, L., Haase, A. T. A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ. J. Vis. Exp. (30), e1561, doi:10.3791/1561 (2009).

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