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A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ
 

A Technique to Simultaneously Visualize Virus-Specific CD8+ T Cells and Virus-Infected Cells In situ

Article DOI: 10.3791/1561-v • 20:48 min • August 13th, 2009
August 13th, 2009 •
1, 2, 1, 1
1Department of Microbiology, Medical School, University of Minnesota, 2Department of Veterinary and Biomedical Sciences, University of Minnesota

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A technique combining in situ tetramer staining and in situ hybridization (ISTH) enables visualization, mapping and analysis of the spatial proximity of virus-specific CD8+ T cells to their virus-infected targets, and determination of the quantitative relationships between these immune effectors and targets to infection outcomes.

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Virus-specific CD8+ T Cells Virus-infected Cells Visualize In Situ Immune Effector Cells Immune Defenses Infection Spatial Proximity Quantitative Relationships Clearance Chronic Persistent Infections In Situ Tetramer Staining In Situ Hybridization Viral-RNA+ Cells Battle Between Immune Defenses And Infection ISTH (in Situ Tetramer Staining And In Situ Hybridization) Mapping Of Locations E:T Ratios Timing And Magnitude Of The Immune Response
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