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可视化的单分子荧光显微镜下的DNA复制
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생물학
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JoVE 신문 생물학
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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可视化的单分子荧光显微镜下的DNA复制

DOI:
10.3791/1529-v

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15:57 min

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October 09, 2009

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, ,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:11Preparing the DNA Replication Template
  • 02:44Preparing Functionalized Coverslips
  • 05:46Assembling the Flow Chamber
  • 10:03Single-Molecule Replication Experiment
  • 13:18Single Molecule DNA Replication Data Analysis and Representative Results
  • 15:00Conclusion

Summary

자동 번역

자동 번역

此协议表明一个简单的可视化实时个人replisomes的DNA复制的单分子荧光显微镜技术。

Tags

Single-molecule DNA ReplicationFluorescence MicroscopyReal-time MethodCircular DNA TemplateReplication ProteinsNucleotidesGrowing DNA EndReplication RateCompleted DNA ProductsProcessivity Of ReplicationIntercalating DyeFluorescence Microscope

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