Journal
/
Biologie
/
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

22,143 Views

•

15:57 min

•

October 09, 2009

DOI:

10.3791/1529-v

DOI:
10.3791/1529-v

•

15:57 min
October 09, 2009

•

8 Views
, ,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Summary

Automatically generated

This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

Explore More Videos

Single-molecule DNA ReplicationFluorescence MicroscopyReal-time MethodCircular DNA TemplateReplication ProteinsNucleotidesGrowing DNA EndReplication RateCompleted DNA ProductsProcessivity Of ReplicationIntercalating DyeFluorescence Microscope

Read Article

Cite this Article

Tanner, N. A., Loparo, J. J., van Oijen, A. M. Visualizing Single-molecule DNA Replication with Fluorescence Microscopy. J. Vis. Exp. (32), e1529, doi:10.3791/1529 (2009).

Download .ris file

Copy

Share Video

.

Copy