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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
JoVE 杂志
生物学
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JoVE 杂志 生物学
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

22,219 Views

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15:57 min

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October 09, 2009

DOI:

10.3791/1529-v

DOI:
10.3791/1529-v

•

15:57 min
October 09, 2009

•

8 Views
, ,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Summary

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This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.

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Cite this Article

Tanner, N. A., Loparo, J. J., van Oijen, A. M. Visualizing Single-molecule DNA Replication with Fluorescence Microscopy. J. Vis. Exp. (32), e1529, doi:10.3791/1529 (2009).

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