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Ottenere altamente purificato Toxoplasma gondii Oocisti da un gradiente discontinuo di cloruro di cesio
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
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Ottenere altamente purificato Toxoplasma gondii Oocisti da un gradiente discontinuo di cloruro di cesio

13,296 Views

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11:17 min

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November 03, 2009

DOI:

10.3791/1420-v

DOI:
10.3791/1420-v

•

11:17 min
November 03, 2009

•

13 Views
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1Dynamac, Inc., 2Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory,US Environmental Protection Agency

Summary

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Questo studio descrive lo sviluppo di un metodo modificato CsCl che purifica facilmente T. gondii oocisti dalle feci di gatti infetti che sono adatti per biologia molecolare e la cultura manipolazione dei tessuti

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Cite this Article

Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

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