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Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

DOI:
10.3791/1420-v

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11:17 min

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November 03, 2009

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, , ,
1Dynamac, Inc., 2Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory,US Environmental Protection Agency

Chapters

  • 00:00Title
  • 01:05Introduction
  • 01:35General Safety When Working With T. gondii Oocysts
  • 03:15Protocol Preparation and the Sucrose Float
  • 07:27CsCl Gradient
  • 10:03Resulting Oocysts
  • 10:34Conclusion

Summary

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This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation

Tags

Toxoplasma GondiiOocystsCesium Chloride GradientProtozoan PathogenFood-borne Disease OutbreaksFeline SpeciesSexually ReplicationInfectious OocystContaminated MeatContaminated SoilContaminated WaterLatent InfectionToxoplasmic EncephalitisImmunocompromisedSeroprevalenceWaterborne Outbreaks

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