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Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient

13,324 Views

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11:17 min

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November 03, 2009

DOI:

10.3791/1420-v

DOI:
10.3791/1420-v

•

11:17 min
November 03, 2009

•

13 Views
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1Dynamac, Inc., 2Department of Biological Sciences,University of Cincinnati, McMicken College of Arts and Science, 3Animal Parasitic Disease Laboratory,Agricultural Research Service, U.S. Department of Agriculture, 4National Exposure Research Laboratory,US Environmental Protection Agency

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This study describes the development of a modified CsCl method that easily purifies T. gondii oocysts from feces of infected cats that are suitable for molecular biological and tissue culture manipulation

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Toxoplasma GondiiOocystsCesium Chloride GradientProtozoan PathogenFood-borne Disease OutbreaksFeline SpeciesSexually ReplicationInfectious OocystContaminated MeatContaminated SoilContaminated WaterLatent InfectionToxoplasmic EncephalitisImmunocompromisedSeroprevalenceWaterborne Outbreaks

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Staggs, S. E., See, M. J., Dubey, J. P., Villegas, E. N. Obtaining Highly Purified Toxoplasma gondii Oocysts by a Discontinuous Cesium Chloride Gradient. J. Vis. Exp. (33), e1420, doi:10.3791/1420 (2009).

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