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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

18,594 Views

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25:06 min

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September 24, 2009

DOI:

10.3791/1333-v

DOI:
10.3791/1333-v

•

25:06 min
September 24, 2009

•

5 Views
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1Departments of Pathology and Cell Biology, and Neuroscience,Columbia University College of Physicians and Surgeons, 2Department of Ophthalmology,Columbia University College of Physicians and Surgeons

Summary

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Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.

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Petros, T. J., Rebsam, A., Mason, C. A. In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells. J. Vis. Exp. (31), e1333, doi:10.3791/1333 (2009).

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