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In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells
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September 24, 2009
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Here we present two techniques for manipulating gene expression in murine retinal ganglion cells (RGCs) by in utero and ex vivo electroporation. These techniques enable one to examine how alterations in gene expression affect RGC development, axon guidance, and functional properties.
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Petros, T. J., Rebsam, A., Mason, C. A. In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells. J. Vis. Exp. (31), e1333, doi:10.3791/1333 (2009).
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