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December 18, 2008
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この手順では、刺激により活発に分泌するIL - 17マウスTh17細胞の白血球の検出と分離を説明します。
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DetectionIsolationViableMouseIL-17-secreting T CellsMACS Cytokine Secretion AssaySingle Cell LevelSensitive IsolationCytokine-secreting CellsIL-17-secreting CellsSingle Cell SuspensionMouse SplenocytesPMA/ionomycinCytokine SecretionIL-17 Catch ReagentBi-specific AntibodyCD45LeukocytesIL-17Secretion RestartTemperature IncreaseTrapped IL-17Flow CytometryAnti-Biotin-PE Antibody
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Foerster, A., Assenmacher, M., Niemoeller, M., Rankin, E., Mohaupt, M., Richter, A. Detection and Isolation of Viable Mouse IL-17-Secreting T Cells. J. Vis. Exp. (22), e1037, doi:10.3791/1037 (2008).
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