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Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
JoVE Journal
Biologia
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JoVE Journal Biologia
Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

DOI:
10.3791/1037-v

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12:38 min

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December 18, 2008

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, , , , ,
1Miltenyi Biotec,GmbH

Capítulos

  • 00:00Título
  • 01:30Introduction
  • 02:10Preparing Reagents
  • 03:07Stimulating the Splenocytes
  • 05:19Labelling Cells with the Catch Reagent
  • 07:36Labeling Cells with IL-17 Detection Antibody (Biotin) and Anti-Biotin-PE
  • 09:50FACS Analysis
  • 11:40Conclusion

Summary

Tadução automática

Tadução automática

This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.

Tags

DetectionIsolationViableMouseIL-17-secreting T CellsMACS Cytokine Secretion AssaySingle Cell LevelSensitive IsolationCytokine-secreting CellsIL-17-secreting CellsSingle Cell SuspensionMouse SplenocytesPMA/ionomycinCytokine SecretionIL-17 Catch ReagentBi-specific AntibodyCD45LeukocytesIL-17Secretion RestartTemperature IncreaseTrapped IL-17Flow CytometryAnti-Biotin-PE Antibody

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