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Detection and Isolation of Viable Mouse IL-17-Secreting T Cells
 

Detection and Isolation of Viable Mouse IL-17-Secreting T Cells

Article DOI: 10.3791/1037-v • 12:39 min • December 18th, 2008
December 18th, 2008 •
1, 1, 1, 1, 1, 1
1Miltenyi Biotec,GmbH

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Resumen
Automatic Translation

This procedure describes the detection and isolation of mouse TH17 leukocytes that actively secrete IL-17 upon stimulation.

Tags

Detection Isolation Viable Mouse IL-17-secreting T Cells MACS Cytokine Secretion Assay Single Cell Level Sensitive Isolation Cytokine-secreting Cells IL-17-secreting Cells Single Cell Suspension Mouse Splenocytes PMA/ionomycin Cytokine Secretion IL-17 Catch Reagent Bi-specific Antibody CD45 Leukocytes IL-17 Secretion Restart Temperature Increase Trapped IL-17 Flow Cytometry Anti-Biotin-PE Antibody
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