JOVE JOURNAL

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CRISPR/Cas9 Gene Editing to Make Conditional Mutants of Human Malaria Parasite P. falciparum

Heather M. Kudyba*^1,2, David W. Cobb*¹, Anat Florentin^1,2, Michelle Krakowiak¹, Vasant Muralidharan^1,2

¹Department of Cellular Biology,University of Georgia, ²Center for Tropical and Emerging Global Diseases,University of Georgia

Journal (Genetics)

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Fani Memi¹, Daniela Tirziu², Irinna Papangeli³

¹Department of Cell and Developmental Biology,University College London, ²Yale Cardiovascular Research Group, Section of Cardiovascular Medicine,Yale School of Medicine, ³Yale Cardiovascular Research Center, Section of Cardiovascular Medicine,Yale School of Medicine

Journal (Genetics)

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Sarah N. Bartolone¹, Maya O. Tree², Michael J. Conway², Michael B. Chancellor^1,3, Laura E. Lamb^1,3

¹Department of Urology,Beaumont Health System, ²Foundational Sciences, College of Medicine,Central Michigan University, ³Oakland University William Beaumont School of Medicine

Journal (Genetics)

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Stefanie L. Morgan*^1,2, Erin Y. Chang*¹, Natasha C. Mariano¹, Abel Bermudez³, Nicole L. Arruda⁴, Fanting Wu⁵, Yunhai Luo¹, Gautam Shankar¹, Star K. Huynh¹, Chiao-Chain Huang⁵, Sharon J. Pitteri³, Kevin C. Wang^1,2,6

¹Department of Dermatology, Program in Epithelial Biology,Stanford University School of Medicine, ²Program in Cancer Biology,Stanford University School of Medicine, ³Canary Center for Cancer Early Detection, Department of Radiology,Stanford University School of Medicine, ⁴Department of Biology,Bridgewater State University, ⁵System Biosciences, ⁶Veterans Affairs Healthcare System

Journal (Genetics)

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms

Clark Fritsch^1,2, Jean-Francois Pierre Gout^3,4, Marc Vermulst¹

¹Center for Mitochondrial and Epigenomic Medicine,Children's Hospital of Philadelphia, ²Department of Cellular and Molecular Biology,University of Pennsylvania, ³Department of Biological Sciences,Mississippi State University, ⁴Center for Mechanisms of Evolution, Biodesign Institute,Arizona State University

Journal (Genetics)

Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes

Athanasios Saragliadis¹, Thomas Trunk¹, Jack C. Leo¹

¹Evolution and Genetics, Department of Biosciences,University of Oslo

Journal (Genetics)

Maintaining Biological Cultures and Measuring Gene Expression in Aphis nerii: A Non-model System for Plant-insect Interactions

Stephanie S.L. Birnbaum¹, David C. Rinker¹, Patrick Abbot¹

¹Biological Sciences Department,Vanderbilt University

Journal (Genetics)

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Erin L. Sorlien¹, Mary A. Witucki¹, Joseph Ogas¹

¹Department of Biochemistry,Purdue University

Journal (Genetics)

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Xuan Guo^1,2, Yiqing Zhao^1,3, Hieu Nguyen¹, Tonghua Liu², Zhenghe Wang^1,3, Hua Lou^1,3,4

¹Department of Genetics and Genome Sciences,Case Western Reserve University, ²Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, ³Case Comprehensive Cancer Center,Case Western Reserve University, ⁴Center for RNA Science and Therapeutics,Case Western Reserve University

Journal (Genetics)

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Amanda Haupt¹, Tanya Grancharova¹, Joy Arakaki¹, Margaret A. Fuqua¹, Brock Roberts¹, Ruwanthi N. Gunawardane¹

¹Allen Institute for Cell Science

Journal (Genetics)

Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations

Alec Kittredge¹, Changyi Ji¹, Yu Zhang ¹, Tingting Yang¹

¹Department of Pharmacology and Physiology,University of Rochester, School of Medicine and Dentistry

Journal (Genetics)

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

Ravinder Singh¹

¹Department of Molecular, Cellular and Developmental Biology,University of Colorado at Boulder

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