JOVE JOURNAL

Genetics

Showing 253 - 264 in 413 videos.

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Journal (Genetics)

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Erin L. Sorlien¹, Mary A. Witucki¹, Joseph Ogas¹

¹Department of Biochemistry,Purdue University

Journal (Genetics)

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Xuan Guo^1,2, Yiqing Zhao^1,3, Hieu Nguyen¹, Tonghua Liu², Zhenghe Wang^1,3, Hua Lou^1,3,4

¹Department of Genetics and Genome Sciences,Case Western Reserve University, ²Department of Endocrinology,Dongfang Hospital of Beijing University of Chinese Medicine, ³Case Comprehensive Cancer Center,Case Western Reserve University, ⁴Center for RNA Science and Therapeutics,Case Western Reserve University

Journal (Genetics)

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Amanda Haupt¹, Tanya Grancharova¹, Joy Arakaki¹, Margaret A. Fuqua¹, Brock Roberts¹, Ruwanthi N. Gunawardane¹

¹Allen Institute for Cell Science

Journal (Genetics)

Differentiation, Maintenance, and Analysis of Human Retinal Pigment Epithelium Cells: A Disease-in-a-dish Model for BEST1 Mutations

Alec Kittredge¹, Changyi Ji¹, Yu Zhang ¹, Tingting Yang¹

¹Department of Pharmacology and Physiology,University of Rochester, School of Medicine and Dentistry

Journal (Genetics)

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates

Ravinder Singh¹

¹Department of Molecular, Cellular and Developmental Biology,University of Colorado at Boulder

Journal (Genetics)

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Naemeh Pourshafie¹, Philip R. Lee², Ke-lian Chen¹, George G. Harmison¹, Laura C. Bott^1,3, Kenneth H. Fischbeck¹, Carlo Rinaldi^1,4

¹Neurogenetics Branch, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Section on Nervous System Development and Plasticity, The Eunice Kennedy Shriver National Institute of Child and Human Development,National Institutes of Health, ³Department of Molecular Biosciences, Rice Institute for Biomedical Research,Northwestern University, ⁴Department of Physiology, Anatomy and Genetics,University of Oxford

Journal (Genetics)

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Wing H. Wong*^1,2, R. Spencer Tong*^1,2, Andrew L. Young^1,2, Todd E. Druley^1,2

¹Department of Pediatrics, Division of Hematology and Oncology,Washington University School of Medicine, ²Center for Genome Sciences and Systems Biology,Washington University School of Medicine

Journal (Genetics)

The Use of Mouse Splenocytes to Assess Pathogen-associated Molecular Pattern Influence on Clock Gene Expression

Adam C. Silver¹

¹Department of Biology,University of Hartford

Journal (Genetics)

Ultralow Input Genome Sequencing Library Preparation from a Single Tardigrade Specimen

Yuki Yoshida^1,2, Sayuri Konno^1,3, Ryousuke Nishino^1,3, Yumi Murai^1,3, Masaru Tomita^1,2,3, Kazuharu Arakawa^1,2,3

¹Institute for Advanced Biosciences,Keio University, ²Systems Biology Program, Graduate School of Media and Governance,Keio University, ³Faculty of Environment and Information Studies,Keio University

Journal (Genetics)

Mating-based Overexpression Library Screening in Yeast

Elliott Hayden¹, Shuzhen Chen¹, Abagail Chumley¹, Quan Zhong¹, Shulin Ju¹

¹Department of Biological Sciences,Wright State University

Journal (Genetics)

High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping

Ceres Kosuta^1,2, Kate Daniel¹, Devon L. Johnstone^1,2, Kevin Mongeon^1,2, Kevin Ban^1,2, Sophie LeBlanc¹, Stuart MacLeod¹, Karim Et-Tahiry², Marc Ekker², Alex MacKenzie¹, Izabella Pena^1,2

¹Children's Hospital of Eastern Ontario Research Institute, ²Department of Biology,University of Ottawa

Journal (Genetics)

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

Adam B. Cornwell¹, Jesse R. Llop¹, Peter Salzman^2,3, Juilee Thakar⁴, Andrew V. Samuelson¹

¹Department of Biomedical Genetics,University of Rochester Medical Center, ²Department of Biostatistics and Computational Biology,University of Rochester Medical Center, ³Non-Clinical Statistics,Bristol-Myers Squibb, ⁴Department of Microbiology and Immunology,University of Rochester Medical Center

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