JOVE JOURNAL

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RNA-based Reprogramming of Human Primary Fibroblasts into Induced Pluripotent Stem Cells

Patrick S. McGrath^1,2,3, Nicole Diette^1,2, Igor Kogut^1,2,3, Ganna Bilousova^1,2,3

¹Department of Dermatology,University of Colorado School of Medicine, Anschutz Medical Campus, ²Charles C. Gates Center for Regenerative Medicine,University of Colorado School of Medicine, Anschutz Medical Campus, ³Stem Cell Biobank and Disease Modeling Core,University of Colorado School of Medicine, Anschutz Medical Campus

Journal (Developmental Biology)

Microfluidic Assay for the Assessment of Leukocyte Adhesion to Human Induced Pluripotent Stem Cell-derived Endothelial Cells (hiPSC-ECs)

Oleh V. Halaidych¹, Francijna van den Hil¹, Christine L. Mummery^1,2, Valeria V. Orlova¹

¹Department of Anatomy and Embryology,Leiden University Medical Center, ²Department of Applied Stem Cell Technologies,University of Twente

Journal (Developmental Biology)

A Mouse Distraction Osteogenesis Model

Masahito Fujio¹, Yusuke Osawa², Masaki Matsushita², Kota Ogisu¹, Shuhei Tsuchiya¹, Hiroshi Kitoh², Hideharu Hibi¹

¹Department of Oral and Maxillofacial Surgery,Nagoya University Graduate School of Medicine, ²Department of Orthopaedic Surgery,Nagoya University Graduate School of Medicine

Journal (Developmental Biology)

High Frequency Ultrasound for the Analysis of Fetal and Placental Development In Vivo

Nicole Meyer¹, Thomas Schüler², Ana Claudia Zenclussen¹

¹Experimental Obstetrics and Gynecology, Medical Faculty,Otto-von-Guericke University, ²Institute of Molecular and Clinical Immunology, Medical Faculty,Otto-von-Guericke University

Journal (Developmental Biology)

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

Cally Xiao^1,2,3, Frank Nitsche⁴, Hisham Bazzi^2,3

¹Graduate Program in Pharmacology and Experimental Therapeutics,University of Cologne, ²Department of Dermatology and Venereology,University Hospital of Cologne, ³Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD),University of Cologne, ⁴Department of General Ecology, Institute for Zoology, Biocenter Cologne,University of Cologne

Journal (Developmental Biology)

Probing Cell Mechanics with Bead-Free Optical Tweezers in the Drosophila Embryo

Claire Chardès*¹, Raphael Clement*¹, Olivier Blanc¹, Pierre-François Lenne¹

¹CNRS, Institut de Biologie du Développement de Marseille,Aix-Marseille Université

Journal (Developmental Biology)

Visualize Drosophila Leg Motor Neuron Axons Through the Adult Cuticle

Wenyue Guan¹, Lalanti Venkatasubramanian², Myungin Baek³, Richard S. Mann², Jonathan Enriquez¹

¹Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, ²Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute,Columbia University, ³Neuroscience Program,NYU School of Medicine

Journal (Developmental Biology)

Visualization of Tangential Cell Migration in the Developing Chick Optic Tectum

Yuji Watanabe¹, Chie Sakuma¹, Hiroyuki Yaginuma¹

¹Department of Neuroanatomy and Embryology, School of Medicine,Fukushima Medical University

Journal (Developmental Biology)

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Heidi Hongisto*¹, Meri Vattulainen*¹, Tanja Ilmarinen¹, Alexandra Mikhailova², Heli Skottman¹

¹BioMediTech Institute, Faculty of Medicine and Life Sciences,University of Tampere, ²Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences,University of Tampere

Journal (Developmental Biology)

Cell Cycle Analysis in the C. elegans Germline with the Thymidine Analog EdU

Zuzana Kocsisova^1,2, Ariz Mohammad², Kerry Kornfeld¹, Tim Schedl²

¹Department of Developmental Biology,Washington University School of Medicine, St. Louis, Missouri, ²Department of Genetics,Washington University School of Medicine, St. Louis, Missouri

Journal (Developmental Biology)

Micromanipulation of Chromosomes in Insect Spermatocytes

Nicolas K.H. Lin¹, Ryder Nance², Jane Szybist², Alan Cheville², Leocadia V. Paliulis³

¹Program in Cell Biology/Biochemistry,Bucknell University, ²Department of Electrical and Computer Engineering,Bucknell University, ³Biology Department,Bucknell University

Journal (Developmental Biology)

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue

Karina Geurtzen¹, Franziska Knopf^1,2

¹CRTD – Center for Regenerative Therapies Dresden,TU Dresden, ²Center for Healthy Aging,TU Dresden

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