JOVE JOURNAL

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Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Kei Miyamoto^1,2, David Simpson^1,2, John B. Gurdon^1,2

¹Wellcome Trust/Cancer Research UK Gurdon Institute,University of Cambridge, ²Department of Zoology,University of Cambridge

Journal (Developmental Biology)

Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix

Nayeli Rodríguez-Fuentes¹, Olivia Reynoso-Ducoing², Ana Rodríguez-Hernández², Javier R. Ambrosio-Hernández², Maria C. Piña-Barba¹, Armando Zepeda-Rodríguez³, Marco A. Cerbón-Cervantes⁴, José Tapia-Ramírez⁵, Luz E. Alcantara-Quintana⁶

¹Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales,Universidad Nacional Autónoma de México (UNAM), ²Depto. of Microbiología y Parasitología, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), ³Depto. Biología Celular y Tisular, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), ⁴Depto de Biología Celular, Facultad de Química,Universidad Nacional Autónoma de México (UNAM), ⁵Depto. Genetica y Biología Molecular,Cinvestav-IPN, ⁶Subd. de Investigación,Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

Journal (Developmental Biology)

In Utero Intra-cardiac Tomato-lectin Injections on Mouse Embryos to Gauge Renal Blood Flow

Christopher C. Rymer^1,2, Sunder Sims-Lucas^1,2

¹Rangos Research Center,Children's Hospital of Pittsburgh of UPMC, ²Division of Nephrology, Department of Pediatrics,University of Pittsburgh School of Medicine

Journal (Developmental Biology)

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Alberto Muñiz¹, Kaini R. Ramesh¹, Whitney A. Greene¹, Jae-Hyek Choi¹, Heuy-Ching Wang¹

¹Ocular Trauma,U.S. Army Institute of Surgical Research

Journal (Developmental Biology)

Live Imaging of Innate Immune and Preneoplastic Cell Interactions Using an Inducible Gal4/UAS Expression System in Larval Zebrafish Skin

Thomas Ramezani¹, Derek W. Laux¹, Isabel R. Bravo¹, Masazumi Tada², Yi Feng¹

¹MRC Centre for Inflammation Research, Queen's Medical Research Institute,The University of Edinburgh, ²Department of Cell & Developmental Biology,University College London

Journal (Developmental Biology)

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Trevor Cerbini¹, Yongquan Luo¹, Mahendra S. Rao², Jizhong Zou¹

¹National Institute of Arthritis, Musculoskeletal and Skin Diseases,National Institutes of Health, ²Q Therapeutics

Journal (Developmental Biology)

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy

Thomas S. Lisse¹, Elizabeth A. Brochu¹, Sandra Rieger¹

¹Davis Center for Regenerative Biology and Medicine,MDI Biological Laboratory

Journal (Developmental Biology)

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Tongguang Wang¹, Elliot Choi¹, Maria Chiara G. Monaco², Eugene O. Major², Marie Medynets¹, Avindra Nath¹

¹Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke,National Institutes of Health, ²Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Journal (Developmental Biology)

Measuring Protein Stability in Living Zebrafish Embryos Using Fluorescence Decay After Photoconversion (FDAP)

Katherine W. Rogers¹, Alexander Bläßle², Alexander F. Schier¹, Patrick Müller²

¹Department of Molecular and Cellular Biology,Harvard University, ²Systems Biology of Development Group,Friedrich Miescher Laboratory of the Max Planck Society

Journal (Developmental Biology)

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

Kellie Cloney¹, Tamara Anne Franz-Odendaal¹

¹Department of Biology,Mount Saint Vincent University

Journal (Developmental Biology)

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation

Khujista D. Haque¹, Atul K. Pandey¹, Matthew W. Kelley², Chandrakala Puligilla¹

¹Department of Pathology and Laboratory Medicine,Medical University of South Carolina, College of Medicine, ²Laboratory of Cochlear Development,NIDCD, NIH

Journal (Developmental Biology)

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Elizabeth R. Zielins*¹, Ruth Tevlin*¹, Michael S. Hu¹, Michael T. Chung¹, Adrian McArdle¹, Kevin J. Paik¹, David Atashroo¹, Christopher R. Duldulao¹, Anna Luan¹, Kshemendra Senarath-Yapa¹, Graham G. Walmsley¹, Taylor Wearda¹, Michael T. Longaker^1,2, Derrick C. Wan¹

¹Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, ²Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

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