JOVE JOURNAL

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Genetic Engineering of Primary Mouse Intestinal Organoids Using Magnetic Nanoparticle Transduction Viral Vectors for Frozen Sectioning

Lingling Xian¹, Lionel Chia^1,5, Dan Georgess², Li Luo¹, Shuai Shuai¹, Andrew J. Ewald^3,4, Linda M.S. Resar^1,4,5,6

¹Department of Medicine, Division of Hematology,Johns Hopkins University School of Medicine, ²Department of Natural Sciences, School of Arts & Sciences,Lebanese American University, ³Department of Cell Biology,Johns Hopkins University School of Medicine, ⁴Department of Oncology,Johns Hopkins University School of Medicine, ⁵Department of Pathology,Johns Hopkins University School of Medicine, ⁶Institute for Cell Engineering,Johns Hopkins University School of Medicine

Journal (Developmental Biology)

Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry

Josiah Fernandez¹, Enrique C Torchia¹

¹Department of Dermatology and Charles C. Gates Center for Regenerative Medicine,University of Colorado Anschutz Medical Campus

Journal (Developmental Biology)

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

Kana Ishimatsu¹, Anna Cha¹, Zach M. Collins¹, Sean G. Megason¹

¹Department of Systems Biology,Harvard Medical School

Journal (Developmental Biology)

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Shawna M. Rotoli¹, Salvatore J. Caradonna¹

¹Department of Molecular Biology,Rowan University, School of Osteopathic Medicine and Graduate School of Biomedical Sciences

Journal (Developmental Biology)

Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging

Darren Wisniewski¹, Sally Lowell¹, Guillaume Blin¹

¹MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences,University of Edinburgh

Journal (Developmental Biology)

Determining Bile Duct Density in the Mouse Liver

Joshua M. Adams^1,2,3, Hamed Jafar-Nejad^1,3

¹Program in Developmental Biology,Baylor College of Medicine, ²Medical Scientist Training Program (MSTP),Baylor College of Medicine, ³Department of Molecular and Human Genetics,Baylor College of Medicine

Journal (Developmental Biology)

Neurogenesis Using P19 Embryonal Carcinoma Cells

Paweł Leszczyński¹, Magdalena Śmiech¹, Aamir S. Teeli¹, Aleksandra Zołocińska², Anna Słysz², Zygmunt Pojda², Mariusz Pierzchała³, Hiroaki Taniguchi¹

¹Department of Experimental Embryology, Institute of Genetics and Animal Breeding,Polish Academy of Sciences, ²Department of Regenerative Medicine,Maria Skłodowska-Curie Institute - Oncology Center, ³Department of Genomics and Biodiversities, Institute of Genetics and Animal Breeding,Polish Academy of Sciences

Journal (Developmental Biology)

Culturing and Measuring Fetal and Newborn Murine Long Bones

Veronica Uribe¹, Alberto Rosello-Diez¹

¹Australian Regenerative Medicine Institute,Monash University

Journal (Developmental Biology)

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Taiki Nakajima¹, Hidetoshi Sakurai², Makoto Ikeya²

¹Department of Life Science Frontiers, Center for iPS Cells Research and Application,Kyoto University, ²Department of Clinical Application, Center for iPS Cells Research and Application,Kyoto University

Journal (Developmental Biology)

Generation of Organoids from Mouse Extrahepatic Bile Ducts

Junya Shiota¹, Nureen H. Mohamad Zaki¹, Juanita L. Merchant^1,2, Linda C. Samuelson^1,2, Nataliya Razumilava¹

¹Departments of Internal Medicine,The University of Michigan, ²Molecular & Integrative Physiology,The University of Michigan

Journal (Developmental Biology)

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

Yena Kim^1,2, Ji Hyeon Ju^1,2,3

¹CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, College of Medicine,The Catholic University of Korea, ²Department of Biomedicine & Health Science, Seoul St. Mary's Hospital, College of Medicine,The Catholic University of Korea, ³Division of Rheumatology, Department of Internal Medicine, Seoul St. Mary's Hospital, College of Medicine,The Catholic University of Korea

Journal (Developmental Biology)

Analysis of Cardiac Chamber Development During Mouse Embryogenesis Using Whole Mount Epifluorescence

Zhentao Zhang^1,2,3, Young-Jae Nam^1,2,3

¹Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University Medical Center, ²Department of Cell and Developmental Biology,Vanderbilt University, ³Vanderbilt Center for Stem Cell Biology,Vanderbilt University

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