Journal
생화학
Expanding the Mass Photometry Analysis Concentration Range with Microfluidics

Expanding the Mass Photometry Analysis Concentration Range with Microfluidics

January 26, 2024

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

01:09 min
•
973 Views

Measuring Biomolecular Interactions with Weaker Affinities Using Mass Photometry and Microfluidics

03:29 min
•
148 Views

Analysis of the Microfluidics Coupled Mass Photometry Data to Investigate Low-Affinity Protein-Protein Interactions

02:06 min
•
154 Views
, , ,
1Refeyn Ltd., 2Adaptix Ltd., 3Physical and Theoretical Chemistry Laboratory, Department of Chemistry,University of Oxford, 4The Kavli Institute for Nanoscience Discovery,University of Oxford

Summary

This protocol combines mass photometry with a novel microfluidics system to investigate low-affinity protein-protein interactions. This approach is based on the rapid dilution of highly concentrated complexes in solution, which enables low-affinity measurements and broadens the applicability of mass photometry.

Article