Journal
/
Biochemistry
/
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biochemistry
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

English

Automatically Generated

1,826 Views

•

11:30 min

•

June 02, 2022

DOI:

10.3791/64029-v

DOI:
10.3791/64029-v

•

11:30 min
June 02, 2022

•

2 Views
, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Summary

Automatically generated

Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.

Explore More Videos

CalcificationVascular Smooth Muscle CellsFluorescent-based DetectionHVSMCSCalcification MediumDAPIRFPBright FieldImagingData Reduction

Read Article

Cite this Article

Jaminon, A. M. G., Rapp, N., Akbulut, A. C., Dzhanaev, R., Reutelingsperger, C. P., Jahnen-Dechent, W., Schurgers, L. J. A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro. J. Vis. Exp. (184), e64029, doi:10.3791/64029 (2022).

Download .ris file

Copy

Share Video

.

Copy