Journal
/
Immunology and Infection
/
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter

English

Automatically Generated

2,932 Views

•

07:44 min

•

August 12, 2021

DOI:

10.3791/62488-v

DOI:
10.3791/62488-v

•

07:44 min
August 12, 2021

•

1 Views
, , , , , , ,
1Ottawa Hospital Research Institute, 2Department of Biochemistry, Microbiology and Immunology,University of Ottawa, 3Department of Biotechnology,University of Tehran

Summary

Automatically generated

The outlined protocol describes the procedure for producing the HiBiT-receptor-binding domain protein complex and its application for fast and sensitive detection of SARS-CoV-2 antibodies.

Explore More Videos

SARS-CoV-2Receptor-binding DomainAntibody DetectionHiBiT-based BioreporterCOVID-19 ResearchHigh-throughput AssayCell CultureTransfectionPassive Lysis Buffer

Read Article

Cite this Article

Rezaei, R., Surendran, A., Singaravelu, R., Jamieson, T. R., Taklifi, P., Poutou, J., Azad, T., Ilkow, C. S. Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter. J. Vis. Exp. (174), e62488, doi:10.3791/62488 (2021).

Download .ris file

Copy

Share Video

.

Copy