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Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing
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Developmental Biology
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JoVE Journal Developmental Biology
Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

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9,788 Views

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09:35 min

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February 25, 2015

DOI:

10.3791/52489-v

DOI:
10.3791/52489-v

•

09:35 min
February 25, 2015

•

6 Views
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1Biomedical Sciences Program,Midwestern University, 2Department of Microbiology & Immunology,Midwestern University

Summary

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Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

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Cite this Article

Rapanan, J. L., Pascual, A. S., Uppalapati, C. K., Cooper, K. E., Leyva, K. J., Hull, E. E. Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing. J. Vis. Exp. (96), e52489, doi:10.3791/52489 (2015).

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