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גישה מדרגית מאוד לביצוע סקרים אקולוגיים של נמטודות קאנורהבדיטיס Selfing
章
- 00:04Introduction
- 00:34Collection Preparation
- 01:21Field Collection
- 02:21Plating Out Field Collections and Isolating Nematodes from Collections
- 04:06Exporting S-Plates from Fulcrum
- 04:41Check for Proliferation on S-Plates
- 05:26Lysis of Isofemale Lines
- 06:22PCR of SSU and ITS2 Sequences
- 07:00Identifying Nematodes and Processing the Collection Data
- 08:04Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST
- 08:40Conclusion
