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A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
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生物学
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JoVE Journal 生物学
A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes

DOI:
10.3791/63486-v

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09:10 min

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March 01, 2022

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, , , ,
1Department of Molecular Biosciences,Northwestern University

章

  • 00:04Introduction
  • 00:34Collection Preparation
  • 01:21Field Collection
  • 02:21Plating Out Field Collections and Isolating Nematodes from Collections
  • 04:06Exporting S-Plates from Fulcrum
  • 04:41Check for Proliferation on S-Plates
  • 05:26Lysis of Isofemale Lines
  • 06:22PCR of SSU and ITS2 Sequences
  • 07:00Identifying Nematodes and Processing the Collection Data
  • 08:04Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST
  • 08:40Conclusion

概要

自動翻訳

自動翻訳

This protocol can be used to perform large-scale ecological surveys of selfing Caenorhabditis nematodes. The primary advantage of this method is the efficient organization and analysis of ecological and molecular data associated with the nematodes collected from nature.

タグ

Caenorhabditis NematodesEcological SurveyMobile Data CollectionCloud-based DatabaseStandardized Data ProcessingSubstrate TemperatureAmbient TemperatureHumidityOP50 BacteriaNematode Isolation

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