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使用脂质单层方法进行电子晶体学研究的样品制备

JoVE Journal
生化学
Author Produced

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JoVE Journal 生化学

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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使用脂质单层方法进行电子晶体学研究的样品制备

3,531 Views

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04:22 min

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November 20, 2021

DOI:

10.3791/63015-v

DOI:

10.3791/63015-v

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04:22 min

November 20, 2021

•

14 Views

Chloe D. Truong^1,2, Dewight R. Williams³, Mary Zhu¹, Joseph Che-Yen Wang⁴, Po-Lin Chiu^1,2

¹School of Molecular Sciences,Arizona State University, ²Center for Applied Structural Discovery, Biodesign Institute,Arizona State University, ³Eyring Materials Center,Arizona State University, ⁴Department of Microbiology and Immunology,The Pennsylvania State University

概要

几十年来，脂质单层一直被用作形成二维（2D）蛋白质晶体的基础，用于结构研究。它们在气水界面上稳定，可作为电子成像的薄支撑材料。在这里，我们介绍了为生物研究准备脂质单层的行之有效的步骤。

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