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Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
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JoVE Journal 免疫学と感染
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

DOI:
10.3791/60723-v

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08:15 min

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January 31, 2020

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, , , , , , , , ,
1Department of Clinical Medicine,Aarhus University, 2Department of Infectious Diseases,Aarhus University Hospital, 3Department of Biomedicine,Aarhus University, 4Department of Gynecology and Obstetrics,Aarhus University Hospital, 5生物学科,University of Nebraska at Omaha

章

  • 00:04標題
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

概要

自動翻訳

自動翻訳

We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification.

タグ

Humanized NOG MiceIntravaginal HIV ExposureHIV InfectionPreclinical HIV ResearchViral StrainsHIV InterventionsCCR5 VariantViral RNA QuantificationCord Blood ScreeningCCR5 Delta 32HIV Exposure Protocol

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