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Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection
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JoVE 杂志 免疫与感染
Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

Humanized NOG Mice for Intravaginal HIV Exposure and Treatment of HIV Infection

DOI:
10.3791/60723-v

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08:15 min

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January 31, 2020

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, , , , , , , , ,
1Department of Clinical Medicine,Aarhus University, 2Department of Infectious Diseases,Aarhus University Hospital, 3Department of Biomedicine,Aarhus University, 4Department of Gynecology and Obstetrics,Aarhus University Hospital, 5Department of Biology,University of Nebraska at Omaha

Chapters

  • 00:04Title
  • 00:50Cord Blood CCR5Δ32 Genetic Variant Screening
  • 01:50Intravaginal HIV Exposure
  • 03:20RNA Extraction, Complementary DNA (cDNA) Synthesis, and Viral RNA Quantification BY Droplet Digital Polymerase Chain Reaction (ddPCR)
  • 04:55Application of Combination Antiretroviral Therapy (cART)-Containing Chow
  • 05:42Results: Representative HIV infection and Treatment DATA
  • 07:13Conclusion

Summary

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We have developed a protocol for the generation and evaluation of a humanized and human immunodeficiency virus-infected NOG mouse model based on stem cell transplant, intravaginal human immunodeficiency virus exposure, and droplet digital PCR RNA quantification.

Tags

Keywords: Humanized NOG MiceIntravaginal HIV ExposureHIV InfectionPreclinical HIV ResearchViral StrainsHIV InterventionsCCR5 VariantViral RNA QuantificationCord Blood ScreeningCCR5 Delta 32HIV Exposure Protocol

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